mouse cxcl1 gro α  (MedChemExpress)

Journal: Journal of Neuroinflammation

Article Title: An IFNγ/CXCL2 regulatory pathway determines lesion localization during EAE

doi: 10.1186/s12974-018-1237-y

Figure Lengend Snippet: IFNγ suppresses CXCL2 expression in neutrophils and monocytes stimulated with CXCR2 or TLR ligands, respectively. a , b Bone marrow neutrophils were cultured in media alone, or with recombinant CXCL2, CXCL2 plus IL-1β, CXCL1, or G-CSF, in the presence or absence of IFNγ. RNA was extracted 1 h later to measure CXCL2 transcript levels via qPCR. Fold induction of CXCL2 in the stimulated neutrophils over unstimulated controls was calculated as 2 −ΔΔCt , using GAPDH as an internal control for normalization. Each symbol represents a neutrophil culture derived from an individual mouse. ( a n = 8 mice, with data pooled from three experiments. b Representative of two experiments with n = 3 mice each). c Bone marrow monocytes were stimulated with LPS in the presence or absence of IFNγ for 4 h. Intracellular CXCL2 protein expression was assessed by flow cytometry (Representative of three experiments with n = 3 mice each)

Article Snippet: For immunofluorescent histology, primary antibodies included rabbit α-glial fibrillary acidic protein (GFAP) (Gibco), rat α-mouse CD45 (IBL-5/15, Millipore), goat α-mouse CXCL2 (R&D Systems), goat α-mouse CXCL1 (R&D Systems), rat α-mouse Ly6G (IA8, BD Biosciences), and hamster α-mouse CD3ε (BD Biosciences).

Techniques: Expressing, Cell Culture, Recombinant, Control, Derivative Assay, Flow Cytometry

Journal: Journal of Neuroinflammation

Article Title: An IFNγ/CXCL2 regulatory pathway determines lesion localization during EAE

doi: 10.1186/s12974-018-1237-y

Figure Lengend Snippet: CXCL1 is expressed by CNS resident cells during EAE. a CXCL1 (red) was detected in the choroid plexus epithelium in brainstem sections of IFNγRKO mice at the onset of aEAE (clinical score 1), and in astrocytes in spinal cord sections of WT mice at the onset of cEAE (clinical score 2), via immunofluorescent histology. Sections were co-stained with antibodies specific for CD45 (green) and GFAP (white), and counterstained with DAPI (blue). b Choroid plexus samples were obtained at the onset of cEAE or aEAE, or from naïve IFNγRKO mice. CXCL1 transcript was quantified by qPCR and normalized to GAPDH ( n = 4–6/group from two experiments)

Article Snippet: For immunofluorescent histology, primary antibodies included rabbit α-glial fibrillary acidic protein (GFAP) (Gibco), rat α-mouse CD45 (IBL-5/15, Millipore), goat α-mouse CXCL2 (R&D Systems), goat α-mouse CXCL1 (R&D Systems), rat α-mouse Ly6G (IA8, BD Biosciences), and hamster α-mouse CD3ε (BD Biosciences).

Techniques: Staining